Uniting the capabilities of state-of-the-art specialty optical fiber, biophotonics, and neurophysiology
Partner 1, Laboratoire PhLAM, is home to the “FiberTechLille” technology platform (https://fibertech.univ-lille.fr). Part of the Equipex FLUX, it disposes of state-of-the-art equipment for fabricating, characterizing, and doing research on specialty optical fiber. PhLAM is the most well-suited environment imaginable for the WP1 of the NAIMA project. FiberTechLille has long-standing experience with the conception of novel optical fiber designs, and NAIMA will follow the well-established route of establishing target designs through numerical simulations, fabrication of the target designs, and optical testing of the fabricated designs.
Partner 2, Institut Fresnel, disposes of a large range of biophotonics facilities, including nonlinear imaging setups with fs/ps laser sources; small-animal handling facilities managed by the MOSAIC group (https://www.fresnel.fr/mosaic). This institution constitutes an excellent frame for WP2 and WP3 of the NAIMA project, particularly in terms of material resources, like ultra-short pulsed lasers suitable for two-photon imaging; but also because it has a history and culture of developing novel and dedicated optical tools for biological applications. Institut Fresnel disposes of a full range of biological sample handling facilities including molecular biology, cell culture, drosophilia culture, and since 2018 also live animal handling facilities to host living mice coming from Partner 3, INMED.
Partner 3, INMED, is implicated in WP3, the lab will validate the use of the flexible lensless endoscope for brain activity mapping. Using calcium imaging, in vitro and in vivo, INMED made seminal contributions to the understanding of how hippocampal function is supported by specific circuits in health and disease. Indeed, INMED pioneered the use of large-scale multineuron calcium imaging combined with online data mining and electrophysiological or optical probing to dissect the organization of neuronal dynamics.